Low Molecular Weight Components Research Articles

The impacts of pullulan soaking on radish seed germination and seedling growth under salt stress.

Pullulan can not only provide a source of organic carbon but also has excellent properties. However, current research is mostly limited to the physical properties of the high molecular-weight components of pullulan, and little is known of the application of its low molecular-weight components. This study was designed to explore the impact of the pre-soaking of radish seeds in a pullulan solution on seed germination and subsequent seedling growth under salt stress conditions. Pullulan soaking was found to enhance the germination rates of radish seeds subjected to salt stress, while also enhancing the aboveground growth of radish seedlings. Pullulan soaking resulted in increases in chlorophyll, soluble protein, and soluble sugar concentrations in the leaves of these seedlings, together with greater peroxidase activity and root activity as well as decreases in Na+ and malondialdehyde concentrations. This provides an important reference for the application of pullulan in plant protection.

Purification of soda lignin

Purity of technical lignin is one of the main obstacles in the utilization of lignin to value-added chemicals, products, and materials. The objective of this study was to investigate and compare single and two stage purification methods for obtaining soda lignin with high purity. Extensive washing and extraction with water was found effective, increasing the abundance of acid insoluble lignin while reducing its ash content. Extraction with organic solvents was conducted with 2-propanol or blends of n-heptane/1-butanol or cyclohexane/acetone. These solvents were shown to have little effect on the total lignin content, as determined by wet-chemical methods. Two-stage treatments (washing with water followed by solvent extraction) were hence not better than single stage water extraction in terms of the lignin purity. Still, selective removal of low molecular weight components after solvent extraction was noted, reducing the overall polydispersity of the lignin. Evaporation at 40 °C also showed little effect, whereas calcination at 150 °C significantly increased the molecular weight of the soda lignin. The latter effect was explained by thermally induced cross-linking. In addition, the UV absorbance of the calcinated lignin increased, which is likely related to changes in the aromatic structure. Such effect also entailed that UV/vis spectrophotometry was found less reliable in determining the total lignin content. At last, a mathematical model was adapted to predict the total lignin content from FTIR spectrometry. In conclusion, the tested procedures can be used to purify soda lignin and adjust its molecular weight.

A cluster approach to rationalize shear thinning: Application to polymer solutions and suspension fluids

This paper calculates for liquid mixtures of high and low molecular weight components, how many solute molecules flow on the average conjointly. The application of the approach to solutions of poly(dimethyl siloxane) in its pentamer, to suspensions of gibbsite (Al(OH)3) in dimethyl sulfoxide, and to human blood testifies that the approach is applicable without restrictions regarding the chemical nature of the high molecular weight compound. The shear thinning of the systems under investigation can be understood in terms of a reduction of the shear-overlap parameter Σ, where the generalized intrinsic viscosity {η} constitutes the central property governing the composition and shear rate dependence of the viscosities. Furthermore, the present analysis demonstrates that intrinsic viscosities can be determined for all solutes and that they decrease with rising shear rates according to a Boltzmann sigmoid for the systems DMS5/PDMS and blood. The comparison of the hydrodynamic specific volumes of the solutes (i.e., of [η]) with the corresponding specific volumes in the pure state leads to the conclusion that solutes that cannot interpenetrate carry a considerable amount of solvent piggyback with them when flowing. In addition to the pure description of the observations, the approach was able to point to new phenomena, e.g., the solidification of the gibbsite suspensions beyond a characteristic solute concentration, which shifts to higher values with increasing shear rates. Because of its general nature, the present approach should become helpful, above all in the areas of technology (reactions in flowing systems) and health (rheology of blood).

Ala®sil chemical characterization and toxicity evaluation: an example of the need for the Medical Device Regulation 2017/745.

Introduction: Ala®sil infusion was on the market for clinical use under the Medical Devices Directive (MDD) 93/42/EEC as an irrigating solution based on polydimethylsiloxane (PDMS). The product was withdrawn in 2016, and to the best of our knowledge, it did not cause any health damage. Methods: A bibliographic review and experimental analysis were conducted to evaluate whether this CE-marked product could have been used in patients under the current Medical Device Regulation (MDR) 2017/745. Analytical results from gas chromatography-mass spectrometry (GC-MS) and matrixassisted laser desorption ionization (MALDI) were performed. Citotoxicity studies were also carried out. Results: Only one study related to Ala®sil clinical use was found, describing a pilot series of five patients. The authors rated the product as not helpful in three out of the five cases for internal searching of retinal breaks and in four out of the five cases for drainage of subretinal fluid. No other scientific papers or documentation was found regarding Ala®sil's safety. Nevertheless, the product was introduced in the market after achieving the CE marking. GC-MS and MALDI showed that the polymer has a low molecular weight of 1,000g/mol. Several linear and cyclic low-molecular-weight components (LMWCs) were identified as impurities ranging from L3 to D8, with a molecular weight below 600g/mol. The Ala®sil sample was found to be cytotoxic after 24h of cell culture but non-cytotoxic after 72h, probably due to the cellular regeneration capacity of an immortalized cell line. Tissular cytotoxicity revealed an increased apoptosis rate but without morphological modifications. Discussion: Although Ala®sil cannot be classified as cytotoxic, this substance appears to increase retinal cell death processes. This study supports the notion that the MDDwas not functioning adequately to ensure the safety of medical devices. However, the current MDR 2017/745 imposes stricter standards to prevent the commercialization of medical devices without high-quality preclinical and clinical information, as well as precise clinical verification for their use, information not available for Ala®sil infusion.

Changes in rheological and low-temperature characteristics of rubberized asphalt containing castor-based bio-oil under thermal-oxidative exposure

The proposed bio-modified rubberized asphalt (BMRA) technology integrates bio-oil and crumb rubber modifications, offering cost savings, waste reuse, improved asphalt performance, and reduced asphalt consumption. Like traditional asphalt, BMRA ages over time due to external environmental factors, impacting its pavement performance. This study examines the alterations in rheological and low-temperature characteristics of BMRA during thermal-oxidative aging. Results indicate that incorporating bio-oil minimizes alterations to the complex modulus and phase angle in aged asphalt. BMRA's crossover modulus significantly surpasses that of unaged rubberized asphalt (RA), showcasing the mitigating effect of bio-oil and crumb rubber on asphalt's rheological properties during aging. BMRA demonstrates comparable or enhanced peak load, fracture displacement, fracture toughness, and fracture energy relative to RA. BMRA's glass transition temperature remains lower than that of other unaged asphalts, signifying enhanced resistance to low-temperature cracking even after aging. Bio-oil, characterized by low molecular weight and abundant light components, counteracts performance deterioration attributed to volatile constituents and destabilization of asphalt's colloid structure during aging. Crumb rubber facilitates the absorption and subsequent release of specific light components, augmenting BMRA's resistance to aging. These findings enhance our understanding of the mechanisms governing the aging resistance and performance of bio-modified rubberized asphalt, thereby supporting the development of sustainable and durable asphalt materials.

High molecular weight bimodal polyethylene elastomers using N,N'-nickel catalysts appended with methoxy and trifluoromethoxy functionality

Polyolefinic materials displaying bimodal molecular weight distributions are of interest since they can potentially provide mechanical toughness from the high molecular weight component without losing processability on account of the low molecular weight component. With this in mind, a series of nickel(II) complexes, [1-[2,6-{(4-MeOC6H4)2CH}2–4-(F3CO)C6H2N]-2-(ArN)C2C10H6]NiBr2 (Ar = 2,4-Me2C6H3Ni1, 2,6-Et2C6H3Ni2, 2,6-iPr2C6H3Ni3, 2,4,6-Me3C6H2, Ni4, 2,6-Et2-4-MeC6H2Ni5, 2,6-Me2C6H3Ni6) have been synthesized and have shown, following treatment with MMAO or Et2AlCl, excellent activity for the polymerization of ethylene (up to 19.87 × 106 g (PE) mol−1 for Ni4/Et2AlCl) generating high molecular weight branched polyethylene. More importantly, the elastomeric material produced using Et2AlCl as activator displays bimodal characteristics as well as low crystallinity and a medium to high branching density. By contrast, the material prepared using MMAO was more unimodal and showed higher crystallinity and lower branching density. Stress–strain and stress–strain recovery tests performed on the bimodal PE’s revealed a range of tensile properties with a sample prepared using Ni5/Et2AlCl combining both high tensile strength and high fracture strength (σ = 20.3 MPa, ε = 892.0 %). Conversely, a PE sample prepared using Ni4/Et2AlCl showed the highest elastic recovery with a stress relaxation (SR) value of 67.6 %. Besides branching analysis, molecular weight determinations and mechanical tests on the polymers, all nickel complexes and precursor 1,2-bis(arylimino)acenaphthenes, have been characterized by a combination of spectroscopic techniques, elemental analysis and in the cases of Ni1(OH2) and Ni4 by single crystal X-ray diffraction. A theory is also proposed to explain the observed bimodality.

Effect of Hyaluronic Acid on the Self-Assembly of a Dipeptide-Based Supramolecular Gel.

The combination of polymers and low molecular weight (LMW) compounds is a powerful approach to prepare new supramolecular materials. Here we prepare two-component hydrogels made by a well-known and biologically active polymer, hyaluronic acid (HA), and a dipeptide-based supramolecular gelator. We undertake a detailed study of materials with different compositions including macroscopic (hydrogel formation, rheology) and micro/nanoscopic characterization (electron microscopy, X-ray powder diffraction). We observe that the two components mutually benefit in the new materials: a minimum amount of HA helps to reduce the polymorphism of the LMW network leading to reproducible hydrogels with improved mechanical properties; the LMW component network holds HA without the need for an irreversible covalent crosslinking. These materials have a great potential for biomedical application as, for instance, extracellular matrix mimetics for cell growth. As a proof of concept, we have observed that this material is effective for cell growth in suspension and avoids cell sedimentation even in the presence of competing cell-adhesive surfaces. This may be of interest to advanced cell delivery techniques.

Evaluation of the Toxicological Effects from Bothrops Jararacussu Snake Venom on Zebrafish (Danio Rerio) Embryos and Larvae

Introduction: Approximately 90% of reported and identified cases of snakebites in Brazil are caused by species belonging to the Bothrops genus. These snakes have clinical rele-vance due to their venom composition, which contains substances capable of triggering local and systemic effects, leading to morbidities and/or mortality. Objective: The objective of this study was to evaluate the toxic and toxinological effects of Both-rops Jararacussu snake venom on zebrafish embryos and larvae. Methods: The stability of B. Jararacussu snake venom under the conditions used in the toxicity experiments in zebrafish embryos and larvae was evaluated on citrated human plasma. Zebrafish embryos and/or larvae mortality, morphological alterations, spontaneous tail movements and heartbeat caused by the venom were quantified within 96 hours. Toxicity parameters and activity of enzyme-related toxicity biomarkers were evaluated in zebrafish after 96 hours of semi-static exposure to the venom. Results: The results indicated that the venom causes toxicity in zebrafish embryos and larvae, in-ducing embryonic mortality, alteration in the number of spontaneous tail movements and activity of biomarker enzymes. The results suggested that the toxic effects caused by the venom in the ear-ly stages of zebrafish development are mediated, in part, by neurotoxic action, induction of oxida-tive and metabolic stress caused by low molecular weight components, and proteins present in this venom. Conclusion: Toxinological evaluations using the zebrafish as a model are scarce; however, this study presented promising results that encourage the development of future research in toxinology using this animal as a model organism.

Characterization of copper binding to different molecular weight fractions of dissolved organic matter in surface water

Dissolved organic matter (DOM) is a kind of substance with complex compositions and wide molecular weight distribution, which can strongly combine with various pollutants. Therefore, the binding characteristics of DOM and heavy metal pollutants can be studied specifically according to the binding characteristics of DOM and pollutants. In this study, DOM in surface water bodies was divided into three levels (MW < 1 kDa, 1 kDa < MW < 5 kDa, MW > 5 kDa) according to different molecular weights (MW). The binding properties were investigated by fluorescence spectrum analysis and complex model. Four components (C1–C4) were identified by PARAFAC. Among them, the contribution rate of protein-like components C1, C2 and C4 to the total fluorescence intensity reached more than 78%, and the log Ka values of low molecular weight components were the highest, which were 3.28, 3.14 and 3.47, respectively, indicating higher binding ability with Cu2+.With the decrease of molecular weight, the log Kb value increases, indicating that the complexation is more stable. The humic component C3 in high molecular weight has stronger binding stability with Cu2+, but the number of binding sites for C3 is 0.36, while that for C2 is 1.51, indicating that its binding sites and binding ability are relatively low. The results showed that the DOM ligand of Cu2+ in surface water showed a certain molecular weight dependence. In addition, different MW DOM lead to different pollution forms. Different properties of DOM ligand combined with Cu2+ were studied in order to control the migration, transformation, bioavailability, morphology and stability of heavy metal pollutants, and to provide theoretical support for the practical application management of surface water pollution control.

Allergen-free extracts from birch, ragweed, and hazel pollen activate human and guinea-pig submucous and spinal sensory neurons.

Non-allergenic, low molecular weight components of pollen grains are suspected to trigger changes in gut functions, sometimes leading to inflammatory conditions. Based on extensive neuroimmune communication in the gut wall, we investigated the effects of aqueous pollen extracts (APE) on enteric and spinal sensory neurons. Using Ca2+ and fast potentiometric imaging, we recorded the responses of guinea-pig and human submucous and guinea-pig dorsal root ganglion (DRG) neurons to microejection of low (<3 kDa) and high (≥3 kDa) molecular weight APEs of birch, ragweed, and hazel. Histamine was determined pharmacologically and by mass spectrometry (LC-MS/MS). Birch APE<3kDa evoked strong [Ca+2 ]i signals in the vast majority of guinea-pig DRG neurons, and in guinea-pig and human enteric neurons. The effect of birch APE≥3kDa was much weaker. Fast neuroimaging in human enteric neurons revealed an instantaneous spike discharge after microejection of birch, ragweed, and hazel APE<3kDa [median (interquartile range) at 7.0 Hz (6.2/9.8), 5.7 Hz (4.4/7.1), and 8.4 Hz (4.3/12.5), respectively]. The percentage of responding neurons per ganglion were similar [birch 40.0% (33.3/100.0), ragweed 50.8% (34.4/85.6), and hazel 83.3% (57.1/100.0)]. A mixture of histamine receptor (H1-H3) blockers significantly reduced nerve activation evoked by birch and ragweed APEs<3kDa , but was ineffective on hazel. Histamine concentrations in ragweed, birch and hazel APE's < 3 kDa were 0.764, 0.047, and 0.013 μM, respectively. Allergen-free APEs from birch, ragweed, and hazel evoked strong nerve activation. Altered nerve-immune signaling as a result of severe pollen exposure could be a pathophysiological feature of allergic and non-allergic gut inflammation.

Preparation of cellulose tri-acetate membranes for high-alcohol beverages via genetic algorithms and high throughput experimentation

In fermented beverages, volatile esters are trace compounds with a significant effect on the flavour profile. Some of the most important esters in beer are ethyl acetate (solvent-like aroma) and isoamyl acetate (banana aroma). These esters have a defined threshold which, when exceeded, will be experienced as unpleasant upon tasting. Therefore, the industry needs to selectively remove and fractionate esters by means of adsorption and tune the level of esters in the different process streams.Nanofiltration (NF) and Reverse Osmosis (RO) membranes are known to have a high potential towards removal of low molecular weight components from liquid streams. However, membrane preparation depends on several parameters with complex interactions to obtain the targeted membrane performance. Multi-parameter optimization strategies are thus very useful to reduce material consumption, minimize time and labour during the development of a ‘bottom-up’ preparation method. High throughput (HT) filtration techniques in combination with genetic algorithms (GAs) are known to be very powerful to direct such membrane preparation.This optimization strategy is used to obtain cellulose tri-acetate (CTA) membranes for filtration of high-alcoholic beverages towards ethyl acetate and isoamyl acetate depletion. Before the implementation of the proposed strategy, a successful optimization of the HT operating conditions was setup together with a selection of benchmark membranes by screening the performance of various commercially available nanofiltration (NF) and reverse osmosis (RO) membranes.Next, the non-compositional (solvent impregnation, membrane thickness, solvent evaporation time, annealing time and temperature) and compositional parameters (polymer concentration, solvents and additives selection) used during non-solvent induced phase separation (NIPS) preparation of CTA membranes were determined. Finally, CTA membranes were optimized using GAs.

UV/H2O2/O3 removal efficiency and characterization of algae-derived organic matter and odorous substances

Algal organic matter (AOM) released from the metabolic processes of alga is an important source/precursor for toxic substances, thus it has aroused major environmental concerns due to its threat to safe water supplies. In this study, the algae Anabaena was selected as the target species, and a UV/H2O2/O3 advanced oxidation process (AOP) was used for AOM degradation. Overall, the intracellular organic matter (IOM) of Anabaena was mainly composed of soluble microbial metabolites, tyrosin and tryptophan, while the extracellular organic matter (EOM) mainly contained fulvic-acid and humic-acid substances, soluble microbial metabolites. The AOM mainly contained odorous substances such as cyclocitral, ionone, isophorone and a small amount of geosmin (GSM). Low molecular weight and hydrophobic components produced the highest formation potential of trihalomethane (THMFP) and dichloroacetonitrile (DCANFP). The Trichloromethane formation potential (TCMFP) of the different molecular weight (MW) components was higher than the standard for drinking water (> 60 μg/L), while the cytotoxicity of DCANFP was much higher than that of THMFP. The degradation efficiency of the UV/H2O2/O3 technology for cyclocitral was significant, but it could not effectively reduce the TCMFP and DCANFP. After treatment with UV/H2O2/O3, the EEM intensity of organic matter in each area decreased, and the biodegradability of AOM improved.

Improved Cell-Free Transcription–Translation Reactions in Microfluidic Chemostats Augmented with Hydrogel Membranes for Continuous Small Molecule Dialysis

Increasing the protein production capacity of the PURE cell-free transcription-translation (TX-TL) system will be key to implementing complex synthetic biological circuits, and to establishing a fully self-regenerating system as a basis for the development of a synthetic cell. Under steady-state conditions, the protein synthesis capacity of the PURE system is likely at least one order of magnitude too low to express sufficient quantities of all PURE protein components. This is in part due to the fact that protein synthesis cannot be sustained during the entire dilution cycle, especially at low dilution rates. We developed a microfluidic chemostat augmented with semipermeable membranes that combines steady-state reactions and continuous dialysis as a possible solution to enhance protein synthesis at steady-state. In batch operation, the continuous dialysis of low molecular weight components via the membranes extended protein synthesis by over an order of magnitude from 2 h to over 30 h, leading to a 7-fold increase in protein yield. In chemostat operation, continuous dialysis enabled sustained protein synthesis during the entire dilution cycle even for low dilution rates, leading to 6-fold higher protein levels at steady state. The possibility to combine and independently manipulate continuous dialysis and chemostat operation renders our dialysis chemostat a promising technological basis for complex cell-free synthetic biology applications that require enhanced protein synthesis capacity.

Activity of low-molecular weight components of Larix sibirica antioxidant system under exposure to technogenic pollution.

Changes in the antioxidant protection system of Larix sibirica Ledeb at different pollution levels caused by emissions from a large aluminum smelter (BrAS) have been studied. We revealed that the content of peroxide (H2O2) in the needles is a reliable marker of oxidative stress in the trees under pollution. The crucial role of non-enzymatic components, in particular, proline, phenolic compounds, ascorbic acid, glutathione, in reducing the level of free radicals in the needles cells was found. Proline concentration in the needles significantly rises with the increase in pollution levels from low to high. Under critical level pollution, it decreases by 40% compared to the background. The total content of ascorbic acid (ASC) in the needles of polluted trees varies slightly; however, there are significant changes in its various forms. With an increase in pollution to a high level, the content of the reduced form of ASC in the needles increases by 1.5-2.9 times compared to the background content. At a critical level of pollution, the total level of ascorbic acid and its reduced form falls, the content of the oxidized form reaches minimum values. The total content of phenolic compounds in the needles increased by 50-55%, concentration of flavonoids by 1.5-1.8 times, catechins by 1.9-2.5 times, proanthocyanidins by 45% compared to the background level under low, moderate, high pollution, whereas under critical pollution their content decreased. The absolute concentration of the reduced form glutathione in the needles falls by 1.9-3.0 times, the oxidized form increases by 1.5-2.0 times compared to the background. The ratio of reduced glutathione to oxidized glutathione decreased, especially during critical pollution. The data obtained show significant activation of Siberian larch biochemical protection at low, moderate and high levels of pollution by the aluminum smelter emissions. At a critical levels of contamination, a significant depletion of the pool of low-molecular antioxidants was observed.

Fouling evolution of extracellular polymeric substances in forward osmosis based microalgae dewatering

Membrane fouling was still a challenge for the potential application of forward osmosis (FO) in algae dewatering. In this study, the fouling behaviors of Chlorella vulgaris and Scenedesmus obliquus were compared in the FO membrane filtration process, and the roles of their soluble-extracellular polymeric substances (sEPS) and bound-EPS (bEPS) in fouling performance were investigated. The results showed that fouling behaviors could be divided into two stages including a quickly dropped and later a stable process. The bEPS of both species presented the highest flux decline (about 40.0%) by comparison with their sEPS, cells and broth. This performance was consistent with the largest dissolved organic carbon losses in feed solutions, and the highest interfacial free energy analyzed by the extended Derjaguin–Landau–Verwey–Overbeek (XDLVO) theory. The chemical characterizations of algal foulants further showed that the severe fouling performance was also consistent with a proper ratio of carbohydrates and proteins contents in the cake layer, as well as the higher low molecular weight (LMW) components. Compared with the bEPS, the sEPS was crucial for the membrane fouling of S. obliquus, and an evolution of the membrane fouling structure was found in both species at the later filtration stage. This work clearly revealed the fundamental mechanism of FO membrane fouling caused by real microalgal suspension, and it will improve our understanding of the evolutionary fouling performances of algal EPS.

Characteristics of products of thermal and catalytic cracking of heavy oil asphaltenes under supercritical water conditions

This work deals with the reactivity and structural changes of asphaltenes during cracking in supercritical water using water-soluble salts Co and Ni. Cracking of the asphaltenes was carried out in an autoclave with a volume of 12 cm3 at a temperature of 450 °C, the process time was 80 min and the pressure was ≈ 29–30,8 MPa. The reactivity and structural changes of the asphaltenes were evaluated based on the yield and composition of the products (gas, maltenes, and coke) and the characteristics of the residual asphaltenes obtained by the method of structural group analysis based on elemental analysis, the values of molecular weight and 1H nuclear magnetic resonance (1H NMR), and XRD. Under supercritical water conditions, coke formation reactions are slowed down, with increasing diameter of the carbon stack structure. It is found out that the cobalt-containing catalyst promotes the formation of additional amounts of lower molecular weight components, i.e. maltenes and gas. In this case, the structure of secondary asphaltenes refers to the "island", and the number of packs and the total height in the asphaltene cluster increase.

Characterization of dissolved black carbon and its binding behaviors to ceftazidime and diclofenac pharmaceuticals: Employing the molecular weight fractionation

As the ubiquitous component of the aquatic environment, dissolved organic matter (DOM) readily bind with residual pharmaceutical contaminants (PCs) and influence their environmental behaviors. However, the binding mechanisms between dissolved black carbon (DBC), a vital part of the natural DOM pool, and PCs were poorly researched. In this study, the bulk DBC was divided into four fractions in molecular weight (MW) via an ultrafiltration system, and the properties of DBC and their binding interaction with two kinds of typical PCs (ceftazidime (CAZ) and diclofenac (DCF)) were explored concretely. The results showed that low MW component was the main contributor to bulk DBC, and the aromaticity increased with the increase of MW. The categories of chemical structures and fluorescent substances in different MW DBC were similar. Multispectral techniques showed that the oxygen-enriched compounds in DBC had the higher affinity to CAZ/DCF. The –NH–, –COOH, –NH2 groups in CAZ molecules appeared to form the hydrogen bond with DBC. Fluorescence quenching experiments were analyzed, and the binding mechanisms were specifically expounded from the thermodynamic perspective. The fluorophore of fulvic acid-like compounds (FA) were quenched by both static and dynamic quenching mechanisms, while only static quenching occurred for humic acid-like compounds (HA). For bulk DBC, the hydrogen bond and van der Waals force were the major forces in the HA-CAZ system, while the hydrophobic force made the primary contribution to the HA-DCF system, which might be ascribed to the higher hydrophobic nature of DCF. Notably, with the increase of HA MW, the main binding mode of HA-CAZ/DCF changed from hydrophobic force to hydrogen bond and van der Waals force gradually, which also directly proved that various noncovalent interactions co-driven the binding processes. Our findings are beneficial to better assess the fate of DBC and PCs and the corresponding complexes in the aquatic environment.

Nanofiltration process monitoring and antioxidant activity analysis of skimmed milk samples

Dead-end stirred cell experiments were performed with reconstituted skimmed milk and model solutions of single components (lactic acid). When a low solids concentration is presented with the feed, dead-end filtration with a selected polymeric membrane with cut-off of 300 Da is a viable option to assure a high rejection of the dissolved organic compounds (&gt; 90%), combined with a good transport at moderate pressures (20 bar) with an average flux increasing from 48 Lh−1m−2 at room temperature and over 67 L h−1m−2 at 50°C until reaching a volume reduction of about 5. The DPPH antioxidant activity was effectively improved (~3 fold) with some loss due to fouling, temperature and transmission of low-molecular weight components in the permeates in spite of high rejection in the process, whilst the latter have astoundingly an antioxidant capacity, so each permeate can be divided into time intervals of permeation per sample into aliquotes with different DPPH antioxidant activity. That is further confirmed with statistical analysis where р &gt; 0.05. As conclusion, because the temperature of up to 50°C significantly increases the flux without much effect on the antioxidant activity, such temperatures, dilution and conditions are viable option for large-scale production uses.

Biodegradation of dissolved organic matter and sedimentary organic matter in high arsenic groundwater system: Evidence from lipid biomarkers and compound-specific carbon isotopes

Organic matter plays important roles in arsenic mobility. Whether sedimentary or dissolved organic matter is the predominant electron donors is still on debate. To fill this gap, both sediments and groundwater were collected from the Hetao Basin, a typical arid-semiarid area hosting high arsenic groundwater, to characterize diagnostic biomarkers and compound-specific carbon isotopes of lipids in dissolved organic matter (DOM) and its depth-matched sedimentary organic matter (SOM). Results showed that SOM in both clay and sand layers were mainly sourced from terrestrial higher plants, but sand SOM had additional sources from microbial and petroleum OM. Compound-specific carbon isotope compositions of DOM and SOM showed that δ13C of low molecular weight (LMW) n-alkanes (δ13CL) were more positive than those of high molecular weight (HMW) n-alkanes (δ13CH). The δ13CH in groundwater (δ13CH-DOM) and sediments (δ13CH-SOM) fall in the range of C3 plants, indicating that DOM and SOM had similar origins. More positive δ13CL than δ13CH suggests preferential degradation of LMW compounds in both sediments and groundwater. In comparison with SOM, more microbial-derived OM was observed in groundwater DOM, especially in high‑arsenic groundwater. High arsenic groundwater was accompanied by the low ratio of n-alkanes to hopanes in depth-matched SOM, and the great difference between δ13CL and δ13CH and low terrestrial/microbial ratios (TAR) in groundwater DOM. This indicates that SOM and DOM should be utilized by indigenous bacteria to fuel the reductive dissolution of Fe oxides for arsenic release into groundwater. Furthermore, more abundant LMW compounds and weaker odd-to-even predominance in DOM than those in SOM support that DOM encountered stronger biodegradation. This study highlights that preferential biodegradation of DOM (especially LMW components and petroleum sourced OM) would provide electrons for the reductive dissolution of arsenic-bearing Fe/Mn oxides and thus induce arsenic enrichment in groundwater.

In situ characterization of phase transformations in petroleum pitch by high temperature X-ray diffraction

The phase transformation from isotropic to mesophase of a petroleum-derived pitch was monitored by in situ X-ray diffraction at 410 °C. The kinetics of the transformation were characterized by monitoring the growth of a broad peak at 2θ∼24° and could be described by Avrami’s equation. The volatilization of low molecular weight components originally present in the pitch, or produced during the condensation reaction, was assessed by thermal gravimetry analysis, following the same thermal history. Ex situ characterizations of the pitch precursor before and after thermal treatment provided insight into the evolution of the molecular structure associated with mesophase generation (optical polarized microscopy, molecular weight distribution by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, X-ray photoelectron spectroscopy, Auger spectroscopy, and thermal stability). This investigation demonstrates the value of using complementary in situ and ex situ experimental techniques to characterize complex mechanisms during the processing of organic materials.